The 33 year old R&B pop star will kick off his tour in Melbourne's Etihad Stadium on September 18, followed by Adelaide, Brisbane, Sydney then Perth.
The tour is expected to be hugely popular as internet pre-sales tickets go on sale March 6, with general on-sale to the public beginning March 7.
It will be first time the singer has returned to the country since his "FutureSex/LoveSounds" tour in 2007.
The 20/20 Experience, Timberlake's latest album since the release of FutureSex/LoveSounds in 2006 , has been critically well received and was last year's best selling album with over 4 million sold to date worldwide.
At the time of its release in March 2013, it was the fastest-selling album in iTunes history for a record's opening week, selling over 580,000 digital copies on iTunes worldwide.
The album consists of successful singles "Suit & Tie" and "Mirrors".
Justin Timberlake's '20/20 Experience' Australian tour dates:
Sept. 18 – Etihad Stadium, Melbourne
Sept. 22 – Adelaide Entertainment Centre
Sept. 26 – Brisbane Entertainment Centre
Oct. 1 – Allphones Arena, Sydney
Oct. 8 – Perth Arena